Regulatory

Part:BBa_M36145:Design

Designed by: Jane Hae Soo Shin   Group: Stanford BIOE44 - S11   (2012-06-06)


Cold-inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 142
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 202


Design Notes

This sequence was found in CHO cells. In other types of cell, it is not guaranteed that the promoter will be up-regulated by the lowering of the temperature.


Source

http://ukpmc.ac.uk/articles/PMC3118111/pdf/1472-6750-11-51.pdf

References